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Selection Guide #11

Colour Fastness to Perspiration: ISO 105-E04, AATCC 15 and Which Edition to Cite

Why the result is a grade and not a number, why the ISO method needs two baths, and why AATCC 15 is not half of the kit.

1. What does a colour fastness to perspiration test actually measure?

Colour fastness, spelled color fastness in US usage and the same property either way, means the resistance of a colour to a defined stress: light, washing, rubbing, or in this case perspiration. The result is not a concentration, it is a grade. A specimen is exposed under controlled conditions and then read against a reference: the grey scale for change in shade of the specimen itself, and a second grey scale for staining of the undyed adjacent fabric held against it. Both are scored 1 to 5, where 5 is no perceptible change.

Two consequences follow, and both surprise people arriving from chemical testing. First, the number is ordinal: a 4 is better than a 3, but the interval between them is not a quantity. Second, the adjacent fabric matters as much as the specimen. A dye that holds its own shade perfectly and transfers heavily onto the multifibre strip has failed the test that matters commercially, because in wear that transfer lands on someone's skin or on the garment underneath.

Which stress is named matters, because the grades do not transfer. Colour fastness to washing (the ISO 105-C series) exposes the specimen to detergent, temperature and mechanical action; colour fastness to perspiration (ISO 105-E04) exposes it to a sweat solution under a load at body temperature. A dye can grade 4-5 to washing and 2 to perspiration, and a report that names the wrong one is not a near miss, it answers a different question.

2. Why does ISO 105-E04 need both an acid and an alkaline bath?

Human perspiration is not a single pH. It is acidic when fresh and drifts alkaline as it ages and as bacteria act on it, and dyes do not behave identically across that range: a dye can be perfectly fast in acid and bleed in alkali. That is why ISO 105-E04 runs an acid solution and an alkaline solution as a matched pair, and why a report that cites the standard and shows one bath is incomplete rather than merely brief.

This is the practical reason the two solutions are supplied as a kit rather than as separate bottles. A laboratory that runs the acid bath from one batch and the alkaline bath from another, prepared weeks apart, has introduced a variable into a comparison that is supposed to isolate the dye. The volumes match because the specimens are the same size in both baths.

3. Can an AATCC 15 result be reported against ISO 105-E04?

Both are perspiration colour fastness, both use an artificial sweat, and they are routinely treated as interchangeable by people specifying a test from a purchase order. They are not. The clearest difference for ordering purposes is that AATCC 15, the North American method, works with a single acid solution, at a lower pH than the ISO acid bath, while ISO 105-E04 requires the acid and alkaline pair described above.

The consequence is commercial rather than scientific. A buyer supplying both the European and the US market needs both fluids, and a laboratory that has only the ISO kit cannot produce an AATCC 15 report by using half of it. Where the two methods differ beyond the solution, in specimen assembly, in the load applied and in the conditioning, we would rather point you at your testing laboratory than paraphrase two documents we do not publish.

4. Which edition of the standard are you citing?

This comes up often enough to be worth stating plainly: check the edition before the audit, not during it. ISO 105-E04 has been revised, and a technical file that cites a withdrawn edition invites a question that costs more to answer than to prevent. The same applies to the national adoptions. BS EN ISO 105-E04, NF EN ISO 105-E04 and DIN EN ISO 105-E04 are the British, French and German publications of the same European text: one fluid serves all three, and the prefix tells you which national catalogue the copy came from and nothing else.

The version of this we are asked most often is whether ISO 105-E04:2013 is the one to cite. We will not answer that here with a year, and the reason is the point: whichever year we printed would be right until it was not, and a supplier page is the worst possible place to learn that a standard moved. Check the entry in your national body's catalogue, or ask the laboratory that will sign the report, and record the answer in the file with the date you checked it.

We deliberately do not publish a current edition number on this page. Editions move, a page does not, and a supplier stating the wrong one is worse than a supplier stating none. Your standards body or your testing laboratory holds the answer that is true today.

Both methods, the right fluid for each

The ISO 105-E04 kit supplies the acid and alkaline solutions as a matched pair, in equal volumes, from one batch. The AATCC 15 solution is the single acid formulation at the pH that method specifies. Certificate of Analysis per batch on both.

Perspiration kit, ISO 105-E04 Perspiration solution, AATCC 15

Need this fluid at a pH your method fixes, in a volume above the bottle sizes, or to a specification the catalogue does not carry? Ask a chemist for a quote.

Specimen Certificate of Analysis (CoA)

SYNELYS BIOTECH
Quality Control Laboratory, Toulouse, France

CERTIFICATE OF ANALYSIS

Specimen · issued with every batch
Product: —
Test Standard: —
Batch Number: SYB-2026-0724-A
Manufacturing Date: 2026-07-24
Expiry Date: 2027-01-24
Analyzed ParameterSpecificationMethodResultStatus
…

The results relate only to the batch identified above. Measurements were made with instruments calibrated against certified reference solutions (pH buffers, KCl conductivity standard, class E2 masses) and are recorded in the batch record, which states raw-material lots and the production and control history. Synelys Biotech is not an ISO/IEC 17025 accredited laboratory and makes no claim of metrological traceability to a national standard. This is a specimen: figures are representative of a released batch and are not those of any particular lot.

Quality Visa & Batch Release:Quality manager
Released by, quality control · 2026-07-24
CoA template v2 · 2026-09-04—Page 1 of 1

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Safety Data Sheet (SDS)

Synelys Biotech SAS Quality Control Laboratory · Toulouse, France Regulation (EC) No 1907/2006 (REACH), Annex II, as amended by Regulation (EU) 2020/878

SAFETY DATA SHEET

Product range

Revision 3.0 · 2026-09-06
GHS / CLP CLASSIFICATION: MIXTURE NOT CLASSIFIED AS HAZARDOUS
Not subject to ADR Azide-free (no NaN₃) HS code 3822.90.00
SECTION 1
Identification of the substance/mixture and of the company/undertaking
1.1 Product identifier
Trade name: Simulated body fluids and normative test solutions. Product number: specific to the reference, see the product page. Mixture; no REACH registration number, the mixture is not a registrable substance.
1.2 Relevant identified uses and uses advised against
Identified use: laboratory test reagent, for conformity and development testing to the published method cited on the product page. Professional use in a laboratory only.
Uses advised against: any use in or on humans or animals; any clinical, diagnostic or therapeutic use; use as a cosmetic, a medical device, a food or a feed; supply to the general public.
1.3 Details of the supplier of the safety data sheet
Synelys Biotech SAS
Toulouse, France
hello@synelysbiotech.com
1.4 Emergency telephone number
Company: hello@synelysbiotech.com (office hours).
France, 24/7: ORFILA +33 (0)1 45 42 59 59, index of the national poison centres.
Germany, 24/7: Giftinformationszentrum, regional numbers at bvl.bund.de.
Within the EU: 112.
SECTION 2
Hazards identification
2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008 (CLP): the mixture is not classified. No physical, health or environmental hazard class is met.
2.2 Label elements
No hazard pictogram, no signal word and no hazard statement are required. Precautionary statements: none required. The label carries the product name, the reference standard, the batch number, the volume, the filling date, the best-before date and the statement that the product is for laboratory testing use only.
2.3 Other hazards
The mixture does not meet the PBT or vPvB criteria of Annex XIII and contains no constituent identified as having endocrine disrupting properties under Regulation (EU) 2018/1881. Spillage makes a floor slippery. A container held below its recommended temperature may freeze and split.
SECTION 3
Composition / information on ingredients
3.2 Mixtures
Aqueous mixture. Chemical characterisation: inorganic salts and organic metabolites in purified water. Composition as formulated, per litre of ready-to-use solution:
specific to the reference, see the product page
No constituent is present at or above its generic concentration limit, so the mixture stays unclassified. Water is stated for completeness. No component is an SVHC of the candidate list at or above 0.1 % w/w.
SECTION 4
First aid measures
4.1 Description of first aid measures
General: remove soiled clothing. Show this safety data sheet to the doctor.
After inhalation: not an anticipated route of exposure, the product is a non-volatile aqueous solution. Move to fresh air if an aerosol has been generated.
After skin contact: wash with water and soap. If irritation persists, seek medical advice.
After eye contact: rinse with running water for at least 15 minutes, holding the eyelids apart. Remove contact lenses if present and easy to do. If irritation persists, seek medical advice.
After swallowing: rinse the mouth and drink water. Do not induce vomiting. Seek medical advice if symptoms occur.
4.2 Most important symptoms and effects, both acute and delayed
None expected under the conditions of use. A prolonged skin contact with an acidic formulation may cause transient reddening. No delayed effect is known.
4.3 Indication of any immediate medical attention and special treatment needed
Symptomatic treatment. No specific antidote is known or required.
SECTION 5
Firefighting measures
5.1 Extinguishing media
Suitable: water spray, carbon dioxide, dry powder, alcohol resistant foam. The product itself is not combustible and does not sustain a fire.
Unsuitable: none. Choose the media appropriate to the surrounding fire.
5.2 Special hazards arising from the substance or mixture
None specific. In a fire, evaporation of the water fraction may concentrate the dissolved salts and organic constituents in the residue. Thermal decomposition of the organic fraction can release carbon oxides and, where nitrogen bearing constituents are present, nitrogen oxides and ammonia.
5.3 Advice for firefighters
As in any fire, wear self-contained breathing apparatus and full protective clothing. Do not allow firefighting water to enter drains or watercourses.
SECTION 6
Accidental release measures
6.1 Personal precautions, protective equipment and emergency procedures
Wear the protective equipment of section 8. Beware of a slippery floor. No evacuation or special respiratory protection is required for a laboratory scale spill.
6.2 Environmental precautions
Do not discharge a bulk quantity to a drain, to surface water or to the ground. A laboratory quantity may be flushed with plenty of water where local regulations allow it.
6.3 Methods and material for containment and cleaning up
Contain with an inert absorbent (paper, sand, diatomite, universal binder). Collect into a labelled container and dispose of according to section 13. Rinse the area with water and dry it.
6.4 Reference to other sections
Safe handling: section 7. Personal protective equipment: section 8. Disposal: section 13.
SECTION 7
Handling and storage
7.1 Precautions for safe handling
Normal laboratory practice. Avoid contact with the eyes. Do not eat, drink or smoke where the product is handled. Wash the hands after handling. Close the container after use: an opened container is exposed to microbial contamination, and this product carries no preservative.
7.2 Conditions for safe storage, including any incompatibilities
Storage: specific to the reference, see the product page
Shelf life: specific to the reference, see the product page
Container: specific to the reference, see the product page
Keep away from direct sunlight and from freezing. Do not store with strong oxidisers or strong acids. Do not return an unused portion to the original container.
7.3 Specific end use(s)
Laboratory test reagent. See section 1.2. No exposure scenario is attached: a chemical safety assessment is not required for this mixture.
SECTION 8
Exposure controls / personal protection
8.1 Control parameters
No constituent is present in a quantity that requires an occupational exposure limit to be monitored at the workplace. No DNEL or PNEC is available for the mixture; none is required, no chemical safety assessment being called for.
8.2 Exposure controls
Engineering controls: general laboratory ventilation. A fume hood is not required. An eye wash station must be accessible.
Eye and face protection: safety glasses to EN 166.
Skin protection: nitrile gloves to EN ISO 374-1, minimum thickness 0.11 mm. Breakthrough time is not determined for this mixture and must be confirmed by the glove manufacturer. Laboratory coat.
Respiratory protection: not required.
Environmental exposure controls: see sections 6.2 and 13.
SECTION 9
Physical and chemical properties
9.1 Information on basic physical and chemical properties
Physical state: Liquid, except the kits and the powder formats.
Colour: specific to the reference, see the product page
Odour: Odourless
Melting point / freezing point: Approximately 0 °C, the mixture being predominantly water. Not determined experimentally.
Boiling point or initial boiling point and boiling range: Approximately 100 °C at 1013 hPa. Not determined experimentally.
Flammability: Non-flammable. Aqueous solution with no flammable constituent.
Lower and upper explosion limit: Not applicable.
Flash point: Not applicable.
Auto-ignition temperature: Not applicable.
Decomposition temperature: Not determined.
pH: specific to the reference, see the product page
Kinematic viscosity: Not determined. Comparable to water.
Solubility: Fully miscible with water. Not determined in organic solvents.
Partition coefficient n-octanol/water (log value): Not determined. Not meaningful for a mixture of inorganic salts in water.
Vapour pressure: Approximately 23 hPa at 20 °C, that of water. Not determined experimentally.
Density and/or relative density: Approximately 1.00 to 1.02 g/cm³ at 20 °C. Not determined experimentally.
Relative vapour density: Not determined.
Particle characteristics: Not applicable.
9.2 Other information
No physical hazard class is met: the mixture is neither an oxidiser, nor a self-reactive, pyrophoric, self-heating or water-reactive mixture, nor corrosive to metals. No further safety characteristic is relevant.
SECTION 10
Stability and reactivity
10.1 Reactivity
No reactivity hazard under the recommended conditions of use and storage.
10.2 Chemical stability
Stable within the storage conditions of section 7.2 and up to the stated best-before date. Beyond it the fluid may drift outside the specification without any hazard arising.
10.3 Possibility of hazardous reactions
None known.
10.4 Conditions to avoid
Freezing, prolonged heating, direct sunlight, and an open container.
10.5 Incompatible materials
Strong oxidisers. Strong acids and bases. A chloride bearing fluid attacks unprotected carbon steel and some stainless grades: use inert labware.
10.6 Hazardous decomposition products
None under normal conditions. On thermal decomposition, see section 5.2.
SECTION 11
Toxicological information
11.1 Information on hazard classes as defined in Regulation (EC) No 1272/2008
No toxicological study has been performed on the mixture. The assessment below is derived from the constituents by the calculation method of Regulation (EC) No 1272/2008. It is not a statement that the mixture has been tested.
Acute toxicity: Not classified.
Skin corrosion/irritation: Not classified.
Serious eye damage/eye irritation: Not classified.
Respiratory or skin sensitisation: Not classified.
Germ cell mutagenicity: Not classified.
Carcinogenicity: Not classified.
Reproductive toxicity: Not classified.
STOT-single exposure: Not classified.
STOT-repeated exposure: Not classified.
Aspiration hazard: Not classified.
11.2 Information on other hazards
Endocrine disrupting properties: no constituent is identified as an endocrine disruptor under Regulation (EU) 2018/1881.
Other information: the product is not intended for human or animal use. An acidic formulation may transiently redden the skin on prolonged contact.
SECTION 12
Ecological information
12.1 Toxicity
No data available on the mixture. No constituent is classified as hazardous to the aquatic environment.
12.2 Persistence and degradability
The organic fraction (urea, lactic acid, amino acids, glucose) is readily biodegradable. The inorganic salts are not degradable and dissociate in water. No test has been run on the mixture.
12.3 Bioaccumulative potential
Not expected. The constituents are water soluble and of low molecular mass.
12.4 Mobility in soil
High. A water soluble mixture spreads with soil water.
12.5 Results of PBT and vPvB assessment
The mixture does not meet the criteria of Annex XIII. PBT: no. vPvB: no.
12.6 Endocrine disrupting properties
No constituent is identified as an endocrine disruptor.
12.7 Other adverse effects
A large discharge raises the salinity and the nutrient load of a receiving water. Do not discharge in bulk.
SECTION 13
Disposal considerations
13.1 Waste treatment methods
Product: the waste is not classified as hazardous. A laboratory quantity may go to the drain with plenty of water where local rules allow it. A bulk quantity goes to a licensed waste contractor. A batch that has been in contact with a test specimen may carry released metals and is then handled as the specimen requires, not as this sheet describes.
Waste code: assigned by the waste holder according to the origin of the waste, under Commission Decision 2000/532/EC. We do not assign it, not being the holder.
Packaging: rinse the HDPE or PP container with water; it is then recyclable with the plastics stream.
SECTION 14
Transport information
14.1 UN number or ID number
Not applicable.
14.2 UN proper shipping name
Not applicable.
14.3 Transport hazard class(es)
Not applicable.
14.4 Packing group
Not applicable.
14.5 Environmental hazards
No. Not a marine pollutant.
14.6 Special precautions for user
None. Carry upright and secured. Protect from frost in winter transport.
14.7 Maritime transport in bulk according to IMO instruments
Not applicable.
Not classified as dangerous goods under ADR, RID, ADN, IMDG or IATA. No dangerous goods declaration, no limited quantity marking and no dangerous-goods surcharge apply.
SECTION 15
Regulatory information
15.1 Safety, health and environmental regulations specific for the substance or mixture
Regulation (EC) No 1907/2006 (REACH): no constituent is on the Annex XIV authorisation list, and none is restricted under Annex XVII for this use. No constituent appears on the candidate list of substances of very high concern at or above 0.1 % w/w.
Regulation (EC) No 1272/2008 (CLP): mixture not classified.
Directive 2012/18/EU (Seveso III): not covered.
Regulation (EU) 2019/1148 (explosives precursors): no listed substance.
Regulation (EC) No 273/2004 (drug precursors): no listed substance.
National provisions: none specific. The product is not a cosmetic, a medical device, a biocide, a detergent, a food or a feed, and none of those regimes applies to it.
15.2 Chemical safety assessment
No chemical safety assessment has been carried out for this mixture. None is required: the supplier of a mixture is not obliged to carry one out under Title II of Regulation (EC) No 1907/2006.
SECTION 16
Other information
Abbreviations
ADR/RID/ADN/IMDG/IATA: transport of dangerous goods by road, rail, inland waterway, sea and air. CLP: Regulation (EC) No 1272/2008. DNEL: derived no-effect level. PNEC: predicted no-effect concentration. PBT: persistent, bioaccumulative and toxic. vPvB: very persistent and very bioaccumulative. STOT: specific target organ toxicity. SVHC: substance of very high concern. CAS: Chemical Abstracts Service.
Full text of the hazard statements used
None. No hazard statement applies to this mixture.
Revision
Revision 3.0 of 2026-09-06. This revision rewrites the sheet per product: sections 1, 2, 3, 7, 9, 11 and 16 now carry values specific to this reference rather than to the range. Section 9 is completed to the list of Annex II as amended by Regulation (EU) 2020/878, and section 11 states derived classification rather than an untested conclusion. Supersedes all earlier editions.
Notice
The information above rests on the state of our knowledge on the date of issue. It describes the product with regard to safety requirements and is not a warranty of any property. The user remains responsible for observing the law applicable to their own use, and for the risk assessment of the workplace where the product is handled. This product is for laboratory testing use only, not for use in or on humans or animals.

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